Among 306 randomly selected patients of both sexes attending the urogenital clinics of Tanta University Hospital, the incidence of Chlamydia tracltomatis infection was studied using the direct immunofluorescence test (DIMF), tissue culture method, and the clear view method. The overall incidence of the disease was 7.8 %. There was a higher positivity rate in men (8.5%) than in women (7 %), the difference was statistically nonsignificant. Of all patients, 34 % were asymptomatic. The incidence of the disease was higher in symptomatic patients (8.4%) than in asymptomatic ones (6.7%). In women, the disease has a higher incidence in those who had married at younger age (less than 16 years) as well as those using intrauterine contr;tceptive device. The clear view method which is useful only for diagnosis of chlamydia trachomatis infection in women is not as accurate as the other two methods. The sensitivity and specificity of clear view method were 71 % and 100% respectively in symptomatic women compared to DI:tIF method and the false positive and false negative results were 0% and 29% respectively. In asymptomatic women, the sensitivity and specifIcity of the test were 66.66% and 100% while the false positive and false negative results were 0% and 33.33% respectively.