Objective: To examine and validate microbial diagnostic tests for etiological agent of tonsillo-pharyngitis in the study population and to examine antibiotic sensitivity/resistance of Streptococcus pyogenes to certain antimicrobial medications using the disc diffusion method. Method: The study was conducted on 60 children who suffer from recurrent pharyngitis and 20 control healthy children, selected from paediatric outpatient clinic of Benha University, microbiologic laboratory investigations were performed and throat swab obtained. Result: The results of our study showed that streptococcal pyogenes represents 18,7% of cases. They are sensitive to penicillin, linezolid and vancomycin, resistant to streptomycin and cephalexin. Conclusion: Streptococcus pyogenes is a common bacterial cause of pharyngitis representing 18,7% of cases and need swab and culture for diagnosis to differentiate it from other causes. Streptococcus pyogenes is highly sensitive to linezolid (100%) vancomycin (100%) and penicillin (86,7%), and is highly resistant to streptomycin (100%) and cephalexin (100%)