Idiopathic Membranous Nephropathy (IMN) is a renal-limited autoimmune disease and approximately 80% of MNs. The study aimed to evaluate the role of circulation Anti-PLA2R and anti-THSD7A autoantibodies in the diagnosis and differentiation between primary and secondary MN. The study was conducted on 58 adult patients with biopsy- proven MGN. All were subjected to measurement of Anti-PLA2R1 Enzyme-Linked Immunosorbent Assay and anti-THSD7A was detected by indirect immunofluorescence assay. Among the 58 patients, 79.3% were diagnosed as IMN, 20.7% were SMN. Among IMN patients, 32 patients (69.6%) showed positive anti-PLA2R1 antibodies, 2 patients (4.3%) were positive for anti-THSD7A antibodies and the remaining 12 patients (26.1%) were negative for both types of antibodies. Patients with secondary MN were negative for two antibodies. The IMN patients had lower serum creatinine compared to the secondary MN patients (p=0.017). In conclusion, the study demonstrates that serum circulating autoantibodies against PLA2R is a fast, easy, sensitive, and non-invasiveness test for diagnosis of IMN, the combination between serum anti-THSD7A antibodies and anti-PLA2R antibody, may be more sensitive and specific for diagnosis of IMN.