A new Azoles and Azines as pyrazole, thiazole, oxadiazole, triazole, thiadiazole, pyridazine and phthalazine based on quinolone moiety efficiently synthesized via simple and efficient chemical reactions of quinoline-8-yloxy acetohydrazide and quinolone-8-yloxy methyl oxadiazole acetohydrazide with electrophilic reagents in base medium using simple techniques and reagents. The structures of the new synthesized products were deduced based on their analytical, spectral data and by using the fragmentation pattern as a tool for supporting the chemical structures. In addition, the biological activity of the synthesized compounds as antimicrobial and antifungal were screened.