Out of 50 samples were taken from carcasses of cattle and sheep from abattoir, the minimum number/cm² of S. aureus isolated from the carcasses surfaces were 3.7X10² and 1.3X10²while the maximum numbers were 4.5X104 and2.3X104 with a mean values of 4.1X 10³ and 1.8 X 10³, respectively. Staphylococcus aureus colonization from nasal sampling was identified in 67/242 (27.7%) humans, 18/132 (13.6%) cattle, and 6/161 (3.7%) sheep in abattoirs in Assiut province, with methicillin-resistant S. aureus present in 8 (3.30%) humans, 2 (1.5%) cattle, and 0 (0%) sheep. Staphylococcus intermedius was isolated from 10 (4.1%) humans, 51 (38.6%) cattle, and 9 (5.5%) sheep, with methicillin-resistant S.intermedius detected in 1 (0.4%) human, 6 (4.5%) cattle, and 2 (1.2%) sheep. While Staphylococcus aureus colonization from rectal sampling of cattle and sheep were identified in 5 (3.7%) cattle, and 2 (1.2%) sheep, with absent of methicillin-resistant S. aureus in cattle, and sheep while Staphylococcus intermedius was isolated from 37 (28.03) cattle, and 7 (4.3%) sheep, with methicillin-resistant S.intermedius detected in 4 (3.03%) cattle, and absent in sheep. Decontaminating meat surfaces has been the big concern of meat abattoirs. Thus, intervention strategies have been studied to reduce the level of bacteria on animal's carcass surfaces.