A total 100 suspected samples (80 chicken, 15 ducks, and 5 geese) collected from poultry farms and human houses and throat swabs collected from persons having respiratory manifestations and in contact with infected birds were examined by rapid test and Reverse transcription real time-PCR (rRT-PCR) test for detection of avian influenza virus. Rapid antigen detection test detect avian influenza virus type A in seven out of 100 tracheal swabs collected from poultry while, rRT-PCR detect avian influenza virus H5 in two out of 100 samples collected from poultry and one out of 100 throat swabs collected from human in contact with poultry. Reverse transcription real time-PCR gives accurate result with fresh samples than stored samples.