Out of 100 fecal samples from Cattle, 24 gave positive results to Giardiasis, with infectious rate 24%. And also out of 100 fecal samples from man occupational contacts with cattle in different age and seasons, 32 were positives, with infectious rate 32%. Infectious rate of human Giardiasis among man in different ages in Kaloubia Province revealed that the infectious rate was high in infants (11%) followed by preschool children (10%), then adolescents (8%) and lastly in adults (3%) while infectious rate of human Giardiasis among man in different seasons in Kaloubia Province revealed that the summer season was the highest infectious rate (12%), followed by autumn (10%), then spring (8%), and the lowest infectious rate was in winter (2%). In this assay by using heminested PCR-restriction fragment length polymorphism analysis for the detection and genotyping of Giardia lamblia on the basis of polymorphism in the triose phosphate isomerase (tpi) gene. According the taxonomy of Giardia genotype indicate that the assemblages A and B classified as zoonotic type and also represented result may give us indication to the possibility of zoonotic potential of giardiasis