100 samples of dog's sources and 100 from dog's contacts were examined for isolation and identification of Staphylococcus aureus and Staphylococcus intermedius by different methods of isolation and testing. Incidence rate of gingival samples of the dogs (63%-55%), while double infections by the two strains was 12 (12%). Out of 100 samples obtained from skin of apparently healthy dogs contacts, 32 (32%) contained staph. aureus, and only 24 (24%) contained staph. Intermedius, while double infections by the two strains were 8 (8%). S. aureus was isolated more often from dogs with outdoor habitats than indoor habitats while S. intermedius, which was associated with dogs of indoor habitats than out door habitats. Antimicrobial sensitivity test was done for staph. aureus and staph. intermedius isolated strains to Nitrofurantoin (300 Mg) cephalothin (10 Mg), Erythromycin (15Mg), Streptomycin (30Mg), Gentamycin (10Mg), Pencillin G (10 I.U.), and Oxacillin(20Mg) revealed that staph. intermedius was sensitive to all antimicrobial disc used while staph. aureus was sensitive to Nitrofurantoin (300 Mg) cephalothin (10 Mg), Erythromycin (15Mg), Streptomycin (30Mg), Gentamycin (10Mg), Oxacillin(20Mg) and appear some resistance to Pencillin G (10 I.U.).