50 nasal samples were taken from layers fowl had respiratory disorders from private farm, in Qalyobia Governorate, as well as the 50 sputum samples were collected from the fowls care-takers to detect A.fumigatus infection. The percentage of A.fumigatus isolated from suspected infected fowl by fungal infection were15 (30%). The percentage of A.fumigatus isolated from fowls care-takers were 20 (40%). WBCs counts were found to be carried out 20.0 Thos./UL at time zero, was increased to 30.4 after 10 days during treatment ,then increased to 44.2 Thos./UL after add 10 days daily treatment but the count decreased to 20.0Thos./ UL when the poultry was clinically cured . An excellent results were given by using combination between G-CSF and Amphotericin B for treatment zoonotic Aspergillosis, in layers fowls.