Out of 200 fecal samples from dogs, 72 gave positive results to Amebiasis, with infectious rate 36%. But in case of cats 55 out of 200 examined gave positive results, with infectious rate 27.5%. Out of 400 examined animal pets 127 were positives, with infectious rate 31.75%. Highest infectious rate of human Entamoeba histolytica in Qualubia Governorate was in Shebein el-kanater followed by Kaha, Shubra, Kafr shukr, Tukh, Qualub, then Benha respectively. Also it is noticed that the infectious rate was high in infants (48.6%) followed by preschool children (41.4%), then adolescents (17.1%) and lastly in adults (15.7%). Infectious rate of human Entamoeba histolytica among man in different seasons in Qualubia Governorate revealed that the summer season was the highest infectious rate 35.38%, followed by autumn 27.77%, then spring18.75%, and the lowest infectious rate was in winter 17.39%. Out of 560 examined individuals of both sexes from different age groups, 144 were positives with infectious rate 25.7%, and 89 out of 300 males examined were positive with infectious rate29.6%. 55 out of 260 females examined were with infectious rate 21.15%.