Staff Members - Benha University
Tolemerase Reverse Transcriptase Gene Expression as a Tumor Marker for Hepatocellular Carcinoma
American Journal of Biochemistry and Biotechnology • 2011
Publication Information
Authors
1Amal Abou El-Fadle, 1Naglaa Fathy Al Husseini
1Adel F. Al-Kholy, 1Omnia Al-Said, 2Naglaa Al-Toukhy and 2M. Magdi Atta
Keywords
Hepatocellular carcinoma, Telomerase Catalytic Subunit (hTERT), telomerase reverse,
real time PCR, tumor marker, gene expression, diagnostic test, global health problem,
cancer patients, DNA-protein structures
Journal
American Journal of Biochemistry and Biotechnology
Publisher
Science Publications
Volume
7
Issue
2
Pages
55-62
publication.type
International
Paper Link
Not Available
Supplementary Materials
Not Available
Abstract
Problem statement: Hepatocellular carcinoma will emerge as a major form of malignancy
in the coming decades. The continuing high incidence of hepatocellular carcinoma, suggests that this
disease will continue to represent a global health problem far into the future. Different genes encode
for the various components of the human telomerase complex. These components include the human
Telomerase RNA Component (hTERC) and the Telomerase Catalytic Subunit (hTERT). Correlation
between Telomerase Reverse Transcriptase (hTERT) expression and telomerase activity has been
reported in cancer patients. This work aimed to clarify the significance of human Telomerase
Reverse Transcriptase (hTERT mRNA) as a potential molecular tumor marker for Hepatocellular
Carcinoma (HCC). Approach: The current study included 25 patients of hepatocellular carcinoma
(HCC), 30 patients with liver cirrhosis and 25 age and sex matched individuals with normal
laboratory and Image findings as a control group. hTERT mRNA was measured in plasma by Real
time PCR in all patients samples in comparison with normal healthy controls. Results: The
expression of hTERT mRNA by relative unit was 129.10±27.6 with range (67.72-69.6) Vs
5245.87±2382.48 (2053-12232.90) Vs 92782.76±16158 (61783.25-118596.47) for control Vs
cirrhosis Vs HCC group respectively. The hTERT expression was significantly with 699 and 33 fold
increase in HCC and cirrhosis groups correspondingly when compared to that of controls p
in the coming decades. The continuing high incidence of hepatocellular carcinoma, suggests that this
disease will continue to represent a global health problem far into the future. Different genes encode
for the various components of the human telomerase complex. These components include the human
Telomerase RNA Component (hTERC) and the Telomerase Catalytic Subunit (hTERT). Correlation
between Telomerase Reverse Transcriptase (hTERT) expression and telomerase activity has been
reported in cancer patients. This work aimed to clarify the significance of human Telomerase
Reverse Transcriptase (hTERT mRNA) as a potential molecular tumor marker for Hepatocellular
Carcinoma (HCC). Approach: The current study included 25 patients of hepatocellular carcinoma
(HCC), 30 patients with liver cirrhosis and 25 age and sex matched individuals with normal
laboratory and Image findings as a control group. hTERT mRNA was measured in plasma by Real
time PCR in all patients samples in comparison with normal healthy controls. Results: The
expression of hTERT mRNA by relative unit was 129.10±27.6 with range (67.72-69.6) Vs
5245.87±2382.48 (2053-12232.90) Vs 92782.76±16158 (61783.25-118596.47) for control Vs
cirrhosis Vs HCC group respectively. The hTERT expression was significantly with 699 and 33 fold
increase in HCC and cirrhosis groups correspondingly when compared to that of controls p