Objective: Description of an ultrasound (US)-guided technique for retrobulbar nerve blockade in dromedary camel (Camelus dromedarius) cadavers. Study design: Prospective experimental cadaveric study that was carried out in three phases: Phase I: Anatomical dissection and development of US-guided technique; Phase II: Methylene blue (MB) injection; Phase III: Contrast medium (CM), US-guided injections with computerized tomography (CT) control. Animals: A total of 36 orbits from 18 heads were obtained from18 dromedary cadavers. Methods: Phase I: anatomical dissections were carried out bilaterally, using two heads to determine needle site placement. Phase II: an US-guided, lateral, in-plane approach using one of three volumes of MB (3, 6, or 9 mL) was evaluated in 6 heads (4 orbits per volume tested) to establish the ideal injection volume. Injections of MB that strongly stained all retrobulbar nerves were considered successful, whereas insufficient MB volumes resulted in weak or no nerve staining. Phase III: US-guided retrobulbar injection with contrast medium was carried out using 20 orbits. Computed tomography was performed after each injection trial to determine the accuracy of needle placement and CM dispersal. An injection was judged to be successful when the CT images revealed that the needle was located within the retractor bulbi muscle cone and the CM reached the target nerves at the orbitorotundum and the optic foramina. Results: Only injection of 9 mL of MB stained the target nerves sufficiently, whereas there was no or only weak staining with 3 and 6 mL, respectively. Therefore, 9 mL of CM was used for the US-guided injections in phase III. Subsequent CT scans revealed satisfying CM distribution within the ocular muscle cone in 18 of 20 cases (90% success rate). Conclusions and clinical relevance: Ultrasound-guided retrobulbar injection in dromedary cadavers is feasible. Further research is required to assess its practicality and usefulness in vivo.