Staff Members - Benha University
Genetic Polymorphism of Luteinizing Hormone Receptor Gene in Relation to Fertility of Egyptian Buffalo
Bio Technology: An Indian Journal • 2016
Publication Information
Authors
ASA Sosa, Karima Gh M Mahmoud, MMM Kandiel, HAA Eldebaky, MF Nawito and MEA Abou El Roos
Keywords
Buffalo; Fertility; Ultrasound; LHR; PCR-RFLP; Nucleotide sequences
Journal
Bio Technology: An Indian Journal
Publisher
Not Available
Volume
12
Issue
5
Pages
1-11
publication.type
International
Paper Link
Not Available
Supplementary Materials
Not Available
Abstract
The aim of this study was to detect the genetic polymorphism of luteinizing hormone receptor (LHR) gene and its association with
fertility in Egyptian buffalo using PCR-RFLP and sequencing analysis. A total number of 301 comprised of 100 fertile and 201 nonfertile
buffalo were used. Gynecological examinations (aided by ultrasonography) were carried out twice for two successive weeks.
The genomic DNA was extracted from females and the PCR product was assessed. The percentages of inactive ovary and
endometritis (grad, I) were the most common reproductive problems. The mean uterine horn diameter was largest during follicular
phase followed by luteal phase and inactive ovary (2.67 ± 0.11, 2.28 ± 0.09 and 2.18 ± 0.11 cm, respectively). The PCR product of the
primer specific for LHR gene gave the specific band at size 303 bp.The amplified fragments remained undigested by HhaI
restriction enzymes and all tested buffalo were genotyped as TT. DNA sequence of 259 bp out of the amplified 303 bp was
determined. The sequence alignment of 259 bp of Egyptian buffalo LHR with published sequence (accession number:
XM_006078413.1 and JQ885687.1, Bubalus bubalis) showed that the 259 bp segment possess identities at 100%.
In conclusion, monomorphic pattern for LHR gene is considered a unique feature that may be related to the characteristic species
in buffalo. Therefore, the polymorphisms and interaction with the fertility trait should be the subject for further research with a
bigger number of animals.
fertility in Egyptian buffalo using PCR-RFLP and sequencing analysis. A total number of 301 comprised of 100 fertile and 201 nonfertile
buffalo were used. Gynecological examinations (aided by ultrasonography) were carried out twice for two successive weeks.
The genomic DNA was extracted from females and the PCR product was assessed. The percentages of inactive ovary and
endometritis (grad, I) were the most common reproductive problems. The mean uterine horn diameter was largest during follicular
phase followed by luteal phase and inactive ovary (2.67 ± 0.11, 2.28 ± 0.09 and 2.18 ± 0.11 cm, respectively). The PCR product of the
primer specific for LHR gene gave the specific band at size 303 bp.The amplified fragments remained undigested by HhaI
restriction enzymes and all tested buffalo were genotyped as TT. DNA sequence of 259 bp out of the amplified 303 bp was
determined. The sequence alignment of 259 bp of Egyptian buffalo LHR with published sequence (accession number:
XM_006078413.1 and JQ885687.1, Bubalus bubalis) showed that the 259 bp segment possess identities at 100%.
In conclusion, monomorphic pattern for LHR gene is considered a unique feature that may be related to the characteristic species
in buffalo. Therefore, the polymorphisms and interaction with the fertility trait should be the subject for further research with a
bigger number of animals.