أعضاء هيئة التدريس - جامعة بنها
Effectiveness of Using Brilliant Cresyl Blue Staining for Quality Evaluation and Developmental Competence of Immature and Mature Buffalo Oocytes
GLOBAL JOURNAL OF ANIMAL SCIENTIFIC RESEARCH • 2016
معلومات البحث
المؤلفون
Al-shimaa Al-H. H. El-Naby, Karima Gh.M. Mahmoud, Youssef F. Ahmed, Mahmoud E.A. Abouel-Roos and Gamal A.M. Sosa
الكلمات المفتاحية
Buffalo, oocytes, BCB, embryo development
المجلة العلمية
GLOBAL JOURNAL OF ANIMAL SCIENTIFIC RESEARCH
الناشر
Not Available
المجلد
4
العدد
1
الصفحات
20-26
publication.type
International
رابط البحث
Not Available
المواد المرفقة
Not Available
الملخص
The present study aimed to evaluate the effect of brilliant cresyl blue (BCB) stain on quality and developmental competence of mature and immature buffalo
oocytes. Oocytes were exposed to BCB stain diluted in mDPBS (DPBS with
0.4% BSA) for 60 min at 38.5ºC in a humidified air atmosphere before, after
maturation. The cleavage and blastocyst rates were determined. Results showed
the percentage of BCB+
: BCBˉ in immature and mature buffalo oocytes was
54:45 and 70:30 respectively. In immature oocytes percentage of embryo
cleavage rate was highly significantly (P
oocytes. Oocytes were exposed to BCB stain diluted in mDPBS (DPBS with
0.4% BSA) for 60 min at 38.5ºC in a humidified air atmosphere before, after
maturation. The cleavage and blastocyst rates were determined. Results showed
the percentage of BCB+
: BCBˉ in immature and mature buffalo oocytes was
54:45 and 70:30 respectively. In immature oocytes percentage of embryo
cleavage rate was highly significantly (P