Objective: To improve in vitro embryo production in buffalo by supplementation of L-ascorbic acid during maturation and development (experiment 1) and combination with another antioxidant as cysteamine (experiment 2). Methods: Two experiments were performed, the first one aimed to evaluate the different concentrations (0, 25, 50, 100 µM) of L-ascorbic acid on embryo developmental rate of buffalo oocytes. The L-ascorbic acid was added to the maturation and culture media. In the second experiment, oocytes were cultured in media with two type of antioxidant (ascorbic acid + cysteamine) or ascorbic acid only. Results: There was significant increase in cleavage rate at 25, 50 µM than 100 µM and control group. But, the blastocyst rate was higher at 50 µM ascorbic acid than other concentrations (0, 25, 100 µM). Supplementation of ascorbic acid and cysteamineto maturation and cultured media improved embryo development than ascorbic acid alone. Conclusions: Using of 50 µM L-ascorbic acid during in vitro maturation and development improve the developmental competence of buffalo oocytes, this effect was increase with the presence of cysteamine