| publication name | Evaluation of In Vitro Capacitation of Buffalo Frozen/Thawed Sperm by Different Techniques |
|---|---|
| Authors | AR Elkhawagah, V Longobardi, B Gasparrini, GA Sosa, G Bifulco, MEA Abouelroos, AE Abd el-ghafar, G Campanile |
| year | 2013 |
| keywords | |
| journal | Journal of Reproduction and Infertility |
| volume | 4 |
| issue | 3 |
| pages | 19-28 |
| publisher | IDOS Publication |
| Local/International | International |
| Paper Link | http://idosi.org/jri/4(3)13/1.pdf |
| Full paper | download |
| Supplementary materials | Not Available |
Abstract
Abstract: This study aimed to determine the most reliable method to evaluate capacitation of buffalo frozen/thawed sperm. Frozen/thawed sperm were incubated in TALP medium in absence of capacitating agents (control) and in presence of 10 µg/ml heparin for 2 and 4 hrs. Capacitation was assessed by Trypan blue/Giemsa after lysophosphatidylecholine (LPC) exposure, chlortetracycline (CTC) fluorescence assay and immune-localization of tyrosine phosphorylated protein. Furthermore, we evaluated the effect of heparin on penetration