Staff Members - Benha University
Evaluation of In Vitro Capacitation of Buffalo Frozen/Thawed Sperm by Different Techniques
Journal of Reproduction and Infertility • 2013
Publication Information
Authors
AR Elkhawagah, V Longobardi, B Gasparrini, GA Sosa, G Bifulco, MEA Abouelroos, AE Abd el-ghafar, G Campanile
Keywords
Not Available
Journal
Journal of Reproduction and Infertility
Publisher
IDOS Publication
Volume
4
Issue
3
Pages
19-28
publication.type
International
Paper Link
Open Link
Supplementary Materials
Not Available
Abstract
Abstract: This study aimed to determine the most reliable method to evaluate capacitation of
buffalo frozen/thawed sperm. Frozen/thawed sperm were incubated in TALP medium in
absence of capacitating agents (control) and in presence of 10 µg/ml heparin for 2 and 4 hrs.
Capacitation was assessed by Trypan blue/Giemsa after lysophosphatidylecholine (LPC)
exposure, chlortetracycline (CTC) fluorescence assay and immune-localization of tyrosine
phosphorylated protein. Furthermore, we evaluated the effect of heparin on penetration
buffalo frozen/thawed sperm. Frozen/thawed sperm were incubated in TALP medium in
absence of capacitating agents (control) and in presence of 10 µg/ml heparin for 2 and 4 hrs.
Capacitation was assessed by Trypan blue/Giemsa after lysophosphatidylecholine (LPC)
exposure, chlortetracycline (CTC) fluorescence assay and immune-localization of tyrosine
phosphorylated protein. Furthermore, we evaluated the effect of heparin on penetration