Abstract Gentamycin is antibiotic used against Gram –ve bacteria. It has nephrotoxic effect due to production of reactive oxygen species (ROS). The aim of this study is to determine the protective effect of Taurine and calcium carbonate in combination with gentamycin. This study was conducted on 24 animals which were classified into 4 groups, control (C group), Gentamycin treated group (G-group) which was injected by 80mg/kg/day intraperitoneal. Gentamycin and taurine treated group (G+T group) which received gentamycin as the pervious group and taurine in a dose of 7.5mgkgday. Calcium carbonate treated group (G+ Ca group) received caco3 in a dose of1 g/kg orally in combination with gentamycin in the same used dose. By the end of study biochemical analysis of BUN, creatinine, sodium and potassium were measured. Histopathological examination of the renal tissue was done to demonstrate necrosis of renal tissue. The results cleared nephrotoxic effect of gentamycin as BUN, creatinine and area of necrosis were increased significantly (p<0.05). The effect of both taurine and caco3 was demonstrated but the protective effect of caco3 is more than taurine.