Staff Members - Benha University
Reliability of Fibronectin and P-selectin as Indicators of Vitality and Age of Wounds: An Immunohistochemical Study on Human Skin Wounds
Mansoura J. Forens. Med. Clin. Toxicol. • 2018
Publication Information
Authors
Eslam S. Metwally, Abdelmonem G. Madboly, Amina A. Farag, Taghreed A. AbdelazizHesham A. Farag3
Keywords
Fibronectin,
P-selectin,
Immunohistochemical,
Wound vitality,
Scoring system,
Wound age
Journal
Mansoura J. Forens. Med. Clin. Toxicol.
Publisher
Mansoura University
Volume
26
Issue
2
Pages
83-99
publication.type
Local
Paper Link
Open Link
Supplementary Materials
Not Available
Abstract
Fibronectin and P-selectin are immunohistochemical markers of skin wounds
vitality and age estimation. This study aimed to assess and compare between
fibronectin and P-selectin as markers of vitality and dating of antemortem (AM) and
postmortem (PM) human skin wounds. This study was carried out on 140 human
cases that were classified into three main groups; Group I [90 cases received at the
Emergency Department, Benha University Hospital, Egypt], was sub-classified
according to the wound age: AM1 (12 hours; n =20) and Group III [10 normal
control skin samples]. Immunohistochemical (IHC) staining technique was
performed for all full thickness skin biopsies. The current study confirmed that Pselectin
and fibronectin were good markers of wound vitality and designed a scoring
system based on the increased intensity of the IHC staining in relation to wound
age.The current study concluded that P-selectin is more reliable and specific than
fibronectin as a marker of wound age estimation and vitality
vitality and age estimation. This study aimed to assess and compare between
fibronectin and P-selectin as markers of vitality and dating of antemortem (AM) and
postmortem (PM) human skin wounds. This study was carried out on 140 human
cases that were classified into three main groups; Group I [90 cases received at the
Emergency Department, Benha University Hospital, Egypt], was sub-classified
according to the wound age: AM1 (12 hours; n =20) and Group III [10 normal
control skin samples]. Immunohistochemical (IHC) staining technique was
performed for all full thickness skin biopsies. The current study confirmed that Pselectin
and fibronectin were good markers of wound vitality and designed a scoring
system based on the increased intensity of the IHC staining in relation to wound
age.The current study concluded that P-selectin is more reliable and specific than
fibronectin as a marker of wound age estimation and vitality