Neospora caninum is an obligate intercellular protozoan parasite and considered one of the abortive pathogens in a wide range of ruminants. The present study aimed to investigate the antibodies against N. caninum in camels from three governorates in Egypt to understand the epidemiology of neosporosis and its associated abortion. The study was carried out on 282 camels using commercial enzyme-linked immunosorbent assay (ELISA) kit. The obtained results confirmed the presence of anti-Neospora caninum antibodies in 31 out of 282 camels. Moreover, no significant difference in seroprevalence was observed between different localities under the study and between males and females. On contrary, the number of seropositive camels was increased significantly in adult camels of 8–11 years old (OR = 9.1, 95% CI: 1.1–73.1), during the autumn (OR = 2.3, 95% CI: 0.79–7.1) and mainly in females with history of abortion (OR = 2.8, 95% CI: 1.2–6.5) or in camels raised with dogs (OR = 2.8, 95% CI: 1.1–7.08). The presence of antibodies against N. caninum in camels from Egypt increases the demand for further studies to determine its epidemiological role as a cause of abortion and reduce its economic impacts on camel’s industry