Diagnosis of Brucellosis in Ruminants in Kafr El-Sheikh governorate, Egypt
International Journal of Advanced Research • 2015
Publication Information
Authors
Abdelfattah Selim; Ali Gaber; Abdelmoneim Moustafa
Keywords
Brucella, Ruminant, Serology, PCR
Journal
International Journal of Advanced Research
Publisher
Not Available
Volume
3
Issue
1
Pages
345-350
publication.type
International
Paper Link
Open Link
Supplementary Materials
Not Available
Abstract
Brucelosis is one of the most important diseases affecting animals in most of
the developing countries. The study aimed to serological detection of
Brucellosis in ruminants in Kafr El-Sheikh governorate using RBPT and
BPAT as a screening test and ELISA and CFT as a confirmatory test. The
obtained result showed that the incidence rate was highest in sheep (12%)
followed by cattle (8.4%), goat (6.4%) and finally the lowest incidence rate
was in buffaloes (6.1%). The most infected area was Fuwwa and kafr Elsheikh
in comparison with other districts. Moreover, iELISA is most
sensitive (96.5%) and specific test (98.3%) while BAPT is the lowest
sensitive (83.8%) and specific test (90%). The isolated Brucella strain
bacteriologically identified and confirmed by PCR as B. meltensis biovar 3
the developing countries. The study aimed to serological detection of
Brucellosis in ruminants in Kafr El-Sheikh governorate using RBPT and
BPAT as a screening test and ELISA and CFT as a confirmatory test. The
obtained result showed that the incidence rate was highest in sheep (12%)
followed by cattle (8.4%), goat (6.4%) and finally the lowest incidence rate
was in buffaloes (6.1%). The most infected area was Fuwwa and kafr Elsheikh
in comparison with other districts. Moreover, iELISA is most
sensitive (96.5%) and specific test (98.3%) while BAPT is the lowest
sensitive (83.8%) and specific test (90%). The isolated Brucella strain
bacteriologically identified and confirmed by PCR as B. meltensis biovar 3
Staff Members - Benha University