Brucelosis is one of the most important diseases affecting animals in most of the developing countries. The study aimed to serological detection of Brucellosis in ruminants in Kafr El-Sheikh governorate using RBPT and BPAT as a screening test and ELISA and CFT as a confirmatory test. The obtained result showed that the incidence rate was highest in sheep (12%) followed by cattle (8.4%), goat (6.4%) and finally the lowest incidence rate was in buffaloes (6.1%). The most infected area was Fuwwa and kafr Elsheikh in comparison with other districts. Moreover, iELISA is most sensitive (96.5%) and specific test (98.3%) while BAPT is the lowest sensitive (83.8%) and specific test (90%). The isolated Brucella strain bacteriologically identified and confirmed by PCR as B. meltensis biovar 3