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Diagnosis of Brucellosis in Ruminants in Kafr El-Sheikh governorate, Egypt

International Journal of Advanced Research • 2015
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Publication Information
Authors Abdelfattah Selim; Ali Gaber; Abdelmoneim Moustafa
Keywords Brucella, Ruminant, Serology, PCR
Journal International Journal of Advanced Research
Publisher Not Available
Volume 3
Issue 1
Pages 345-350
publication.type International
Paper Link Open Link
Supplementary Materials Not Available
Abstract
Brucelosis is one of the most important diseases affecting animals in most of
the developing countries. The study aimed to serological detection of
Brucellosis in ruminants in Kafr El-Sheikh governorate using RBPT and
BPAT as a screening test and ELISA and CFT as a confirmatory test. The
obtained result showed that the incidence rate was highest in sheep (12%)
followed by cattle (8.4%), goat (6.4%) and finally the lowest incidence rate
was in buffaloes (6.1%). The most infected area was Fuwwa and kafr Elsheikh

in comparison with other districts. Moreover, iELISA is most
sensitive (96.5%) and specific test (98.3%) while BAPT is the lowest
sensitive (83.8%) and specific test (90%). The isolated Brucella strain
bacteriologically identified and confirmed by PCR as B. meltensis biovar 3