Listeria monocytogenes is one of the most important bacterial pathogens for sheep and goat. It in-fects central nervous system as well as genital system causing meningoencphalitis, nervous signs and abortion. Thirty three sheep blood samples (3 of which showed clinical signs of listeriosis as twisting of neck and moving in circles with torticollis and incoordination) were examined by the real-time PCR for lesteriosis. The brains of the affected cases were examined bacteriologically after death of these animals. The contact animals (30 asymptomatic cases) showed negative results by the real-time PCR. The PCR methods showed positive results with the three samples which collected from 3 symptomatic sheep while culture method gave positive results only with two samples.