This investigation is performed on 100 cows aged from (1-6) years. These animals suffered from the
major symptoms of anaplasmosis as fever (41°C) enlargement lymph node and drop in milk yield, emaciation
and anemia. Blood samples were collected from all animals on EDTA for microscopic examination and PCR for
diagnosis of anaplasmosis.. Anaplasma marginale (A. marginale) was detected by PCR in blood samples
obtained from cattle supposed to be infected. The assay employs primers specific for the gene encoding A.
marginale specific primers derived from major surface protein 5 (msp5) gene. The PCR products for 26 positive
samples were subjected to sequence (Labtechnology, Egypt) and BLAST analysis was used for identification
of the genomic DNA of these parasites. Changes associated with A. marginale in these cattle particular
emphasis