This investigation is performed on 100 cows aged from (1-6) years. These animals suffered from the major symptoms of anaplasmosis as fever (41°C) enlargement lymph node and drop in milk yield, emaciation and anemia. Blood samples were collected from all animals on EDTA for microscopic examination and PCR for diagnosis of anaplasmosis.. Anaplasma marginale (A. marginale) was detected by PCR in blood samples obtained from cattle supposed to be infected. The assay employs primers specific for the gene encoding A. marginale specific primers derived from major surface protein 5 (msp5) gene. The PCR products for 26 positive samples were subjected to sequence (Labtechnology, Egypt) and BLAST analysis was used for identification of the genomic DNA of these parasites. Changes associated with A. marginale in these cattle particular emphasis