Botanical medicine that fights the battle against various types of diseases not only in recent decades but also since the dawn of time, by using plant parts or plant as whole as it has low cost and lack of adverse effects. One of these plants that belongs to the Apocynaceae family is Vinca roseus which become widely distributed in tropical and subtropical areas. The aim of the present study was to investigate the phytochemical analysis of leaves of Vinca roseus growing in Egypt. Qualitative Phytochemical screening revealed the presence of Alkaloids, phenolics, Cardiac glycosides, Anthraquinone glycosides, Tannin, Flavonoids, Oils, Resins, Saponins and Protein. Gallic acid, Gums/Mucilages and Terpenoids were not noticeable. The results indicate that leaves extract of Vinca roseus can be used as a source of potential pharmaceutical agent especially in cancer