Gold nanoparticles (AuNPs) was prepared via simple and green method
by using polysaccharides as reducing and stabilizing agents at the same
time. Hiren we used carboxymethylchitosan (CMCS) as a reducing
agent for gold nanoparticle as well as capping agent. CMCS prepared
based on our previous method by reacting chitosan with
monochloroacetic acid in alkaline medium. AuNPs were prepared by
using different concentrations of carboxymethylchitosan (0.2% w/v,
0.5% w/v and 1% w/v) at 100 oC for 1 hour. CMCS was characterized
by using nitrogen content, carboxyl content and FTIR spectra.
AuNPswas characterized by using UV spectrophotometry and TEM
images. Finally, the cytotoxicity of the prepared AuNPs were evaluated
using cell viability assay from MMT and IC50 values compared with
AuNPs prepared by chemical methods. The results shows that AuNPs
have normal distributed with 15-25 nm particle size and its cytotoxicity
was lowered when prepared by this green method and can use GNPs
safely in contact medical treatment with skin.