Staff Members - Benha University
Real-time assays for detection of Phytophthora spp. and identification of an avr3a gene variant
Journal of Plant Diseases and Protection • 2018
Publication Information
Authors
Shimaa R. T. Tolba, Mariella M. Finetti Sialer, Laura C. Rosso, Mahmoud M. A. Moustafa
Chiara Ruggeri3 • Ibrahim I. S. El-Shawaf1 • Aurelio Ciancio
Keywords
avr3a ITS Molecular beacon Phytophthora infestans Phytophthora parasitica Virulence
Journal
Journal of Plant Diseases and Protection
Publisher
Not Available
Volume
Not Available
Issue
Not Available
Pages
Not Available
publication.type
International
Paper Link
Open Link
Supplementary Materials
Not Available
Abstract
A study was carried out for identification and detection of Phytophthora spp. from soil and plant samples, collected from
Solanaceous crops in Egypt and Italy. The samples were screened with specific and universal primers of Phytophthora 18S,
ITS1–ITS2 and 28S regions, followed by PCR product sequencing. The Phytophthora spp. detected were P. infestans
(Egypt) and P. parasitica (Italy). A molecular beacon probe was also developed based on the avr3a gene of P. infestans to
detect a variant associated with virulence traits. The probe was suitable for avr3a allele identification from P. infestans and
also from P. parasitica PCR products.
Solanaceous crops in Egypt and Italy. The samples were screened with specific and universal primers of Phytophthora 18S,
ITS1–ITS2 and 28S regions, followed by PCR product sequencing. The Phytophthora spp. detected were P. infestans
(Egypt) and P. parasitica (Italy). A molecular beacon probe was also developed based on the avr3a gene of P. infestans to
detect a variant associated with virulence traits. The probe was suitable for avr3a allele identification from P. infestans and
also from P. parasitica PCR products.