Staff Members - Benha University
The ameliorative effect of Saussurea costus root extract supplementation against cyclophosphamide-induced anemia in albino rats
Benha Veterinary Medical Journal • 2023
Publication Information
Authors
Rana Salah, Mohamed E Azab, Randa S Ismail, Abeer A Nafeaa
Keywords
Anemia, Bone marrow, Cyclophosphamide, Myelosuppression, Saussurea costus
Journal
Benha Veterinary Medical Journal
Publisher
Not Available
Volume
44
Issue
2
Pages
30-36
publication.type
Local
Paper Link
Open Link
Supplementary Materials
Not Available
Abstract
The current investigation sought to determine the impact of Saussurea costus (S. costus) root
extract (SCRE) supplementation on cyclophosphamide (CTX)-induced anemia. Forty male
albino rats were separated into four groups (10 rats each): Group I (control), Group II
(SCRE), Group III (CTX), and Group IV (CTX+SCRE). Blood and femoral bones were
collected after the end of the experiment. The following parameters were measured;
hematological parameters, erythrocyte oxidative biomarkers, erythrocyte osmotic fragility,
and plasma and erythrocyte Na+
, K
+
and Mg²+
ions levels. Moreover, the histopathological
investigation was conducted. The results revealed that the CTX significantly reduced all
hematological parameters and bone marrow cellularity. CTX also altered erythrocyte
oxidative biomarkers, shifting the osmotic fragility curve to the right and disrupting plasma
and erythrocyte Na+
, K
+
and Mg²+ ions levels. With the supplementation of SCRE, all these
parameters improved. So, it could be concluded that CTX-induced anemia resulting from
myelosuppression and erythrocyte damage caused by oxidative stress could be mitigated by
Saussurea costus root extract (SCRE).
extract (SCRE) supplementation on cyclophosphamide (CTX)-induced anemia. Forty male
albino rats were separated into four groups (10 rats each): Group I (control), Group II
(SCRE), Group III (CTX), and Group IV (CTX+SCRE). Blood and femoral bones were
collected after the end of the experiment. The following parameters were measured;
hematological parameters, erythrocyte oxidative biomarkers, erythrocyte osmotic fragility,
and plasma and erythrocyte Na+
, K
+
and Mg²+
ions levels. Moreover, the histopathological
investigation was conducted. The results revealed that the CTX significantly reduced all
hematological parameters and bone marrow cellularity. CTX also altered erythrocyte
oxidative biomarkers, shifting the osmotic fragility curve to the right and disrupting plasma
and erythrocyte Na+
, K
+
and Mg²+ ions levels. With the supplementation of SCRE, all these
parameters improved. So, it could be concluded that CTX-induced anemia resulting from
myelosuppression and erythrocyte damage caused by oxidative stress could be mitigated by
Saussurea costus root extract (SCRE).