Background: Frequent and chronic inflammation of the pilosebaceous unit of the hair follicle
characterizes acne vulgaris (AV). It is a disease resulting from the interplay of hereditary and
environmental factors. Objectives: Isolation and identification of gram-positive bacteria from Patients
with AV. Methodology: Our study was done on 50 patients attended to the out-patient clinic of
Dermatology, Venerology and Andrology Department of Benha University Hospital. The contents of
open or closed comedones, papules, pustules, and cystic lesions of acne were sampled. The specimens
were cultivated on blood agar plates and then incubated at 37°C for 24 to 48 hours under both aerobic
and anaerobic conditions. Results: There was bacterial growth on both aerobic and anaerobic
conditions. As staph epidermidis (S.epidermidis) were found in 7o%, staph aureus (S. aureus) were
found in 24%, Micrococcus luteus were found in 4% and Leuconostoc mesentroides were found in 2%.
Conclusion: acne vulgaris is a common disease with multimicrobial causes.