BIOCHEMICAL STUDIES ON SOME ANTIOXIDANT ENZYMES FROM NATURAL SOURCES
• 2019
Publication Information
Authors
Nagwa Ibrahem Abd El-Khalek El-Khyat
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publication.type
Local
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Abstract
The present study aims to evaluate the importance of carrot roots (Daucus carota. L) and garlic cloves (Allium sativum .L ) plants as a tropical one recently cultivated in Egypt. Therefore, it is important to study the chemical and biochemical structure of carrot roots (Daucus carota. L) and garlic cloves (Allium sativum .L ). Also, extraction of catalase and peroxidase enzymes from carrot roots and garlic cloves and evaluation properties and kinetics parameters of the enzymes extracts were carried out.
The chemical determinations included contents of moisture, ash, crude protein, total lipids, crude fiber, total carbohydrates, reducing sugar, non -reducing sugar, total carotenoids, ascorbic acid, total phenols and flavonoids Also, the mineral content of carrot roots(Daucus carota. L) and garlic cloves(Allium sativum .L ) were determined. The results of carrot roots and garlic cloves indicated that the moisture content was found to be (87.4. and 59 %), ash (1.11and 7.21% ), crude protein (1.00 and 7.52%), total lipids (0.53and 0.36%) , crud fiber (1.63 and 1.04%) , total carbohydrates (8.33 and 24.87%), reducing sugar (2.11and 0.75%) , non- reducing sugar (1.13and 1.64%), total carotenoids (5.06and 8.29mg/100g), ascorbic acid (8.13and 31mg/100g),respectively. The minerals included the macro minerals K(220.06 and 404.00),P(51.88and 18.54),Na (39.12 and 4.11), Ca (32.15 and 35.56 )and Mg (7.20 and 3.93 ). and micro minerals Fe (2.10 and 8.51 ),Mn(1.43 and 0.001)and Cu,Zn and Pb not reported.
The chemical determinations included contents of moisture, ash, crude protein, total lipids, crude fiber, total carbohydrates, reducing sugar, non -reducing sugar, total carotenoids, ascorbic acid, total phenols and flavonoids Also, the mineral content of carrot roots(Daucus carota. L) and garlic cloves(Allium sativum .L ) were determined. The results of carrot roots and garlic cloves indicated that the moisture content was found to be (87.4. and 59 %), ash (1.11and 7.21% ), crude protein (1.00 and 7.52%), total lipids (0.53and 0.36%) , crud fiber (1.63 and 1.04%) , total carbohydrates (8.33 and 24.87%), reducing sugar (2.11and 0.75%) , non- reducing sugar (1.13and 1.64%), total carotenoids (5.06and 8.29mg/100g), ascorbic acid (8.13and 31mg/100g),respectively. The minerals included the macro minerals K(220.06 and 404.00),P(51.88and 18.54),Na (39.12 and 4.11), Ca (32.15 and 35.56 )and Mg (7.20 and 3.93 ). and micro minerals Fe (2.10 and 8.51 ),Mn(1.43 and 0.001)and Cu,Zn and Pb not reported.
Staff Members - Benha University