Staphylococcus aureus is considered one of the Zooanthroponotic diseases that
normally colonize skin and mucus membrane of human and can also spread from human to
animals by direct or indirect contact during milking and causes disease to animal and human.
The occurrence of S.aureus was determined by using conventional cultural techniques and
biochemical identification. A total of 304 samples were collected from different dairy farms,
237 animal samples collected from (66 cows and 13 buffaloes) including 3 samples (milk
sample, teat swab and nasal swab) from each animal. In addition to 15 samples were collected
from dairy utensils (6 Hand and 9 machine milking). As well as 52 samples from dairy
workers (26 nasal swabs and 26 hand swabs).The results of nasal swabs were 3/66(4.55%) and
2/13(15.38%) from cattle and buffaloes respectively, and teat swabs were 4/66(6.06%)
and2/13(15.38%) from cattle and buffaloes respectively. Also, 5/66(7.58%) of cow’s milk
were positive for S.aureus. Moreover, the results of machine and hand milking dairy utensils
were 11.11% and 50%respectively. The results of dairy worker’s hand and nasal swabs were
23.08% and 15.38%.S.aureus isolates (n=30) showed resistance against amoxicillin/clavulanic
acid (96.7%), ampicillin (90%), and cefotaxime (53.3%).and greater sensitivity to levofloxacin
(100%), ciprofloxacin (70%), erythromycin and azithromycin (both 56.7%), ceftriaxone
(26.7%). The risk of contamination of the milk increased by contaminated hand workers, dirty
utensils with poor hygiene of the farm.