PCR/RFLP of Human growth hormone gene was used to differentiate among ten athletes. Results: AatI, AviII, BsaI, HindII, and SacI restriction endonucleases gave high levels of intraspecific profile for all the studied gene of the ten athletes. Other enzymes including AvaI, Age and DraI were tested for their ability to differentiate these individuals. Conclusion: The study demonstrated that the HGH gene contains useful genetic markers for the identification of athletes.