| publication name | Effects of secondary carbon supplement on biofilm-mediated biodegradation of naphthalene by mutated naphthalene 1, 2-dioxygenase encoded by Pseudomonas putida strain KD9 |
|---|---|
| Authors | Kunal Dutta, Sergey Shityakov, Ibrahim Khalifa, Arpan Mal, Satya Priya Moulik, Amiya Kumar Panda, Chandradipa Ghosh |
| year | 2018 |
| keywords | biodegradation |
| journal | Journal of hazardous materials |
| volume | 357 |
| issue | Not Available |
| pages | 187-197 |
| publisher | Elsiver |
| Local/International | International |
| Paper Link | https://www.sciencedirect.com/science/article/abs/pii/S0304389418303728 |
| Full paper | download |
| Supplementary materials | Not Available |
Abstract
Polycyclic aromatic hydrocarbons (PAHs) belong to a diverse group of environmental pollutants distributed ubiquitously in the environment. The carcinogenic properties of PAHs are the main causes of harm to human health. The green technology, biodegradation have become convenient options to address the environmental pollution. In this study, we analyzed the biodegradation potential of naphthalene with secondary carbon supplements (SCSs) in carbon deficient media (CSM) by Pseudomonas putida strain KD9 isolated from oil refinerary waste. The rigid-flexible molecular docking method revealed that the mutated naphthalene 1,2-dioxygenase had lower affinity for naphthalene than that found in wild type strain. Moreover, analytical methods (HPLC, qRT-PCR) and soft agar chemotaxis suggest sucrose (0.5 wt%) to be the best chemo-attractant and it unequivocally caused enhanced biodegradation of …