Staff Members - Benha University
Changes in Serum and Ascitic Interleukin-10 levels in Cirrhotic Egyptian Patients with Spontaneous Bacterial Peritonitis
• 2016
Publication Information
Authors
Gehad Ragab Mahmoud ELshawarbi, Ashraf Khames Nassar, Maha Zein ELabedin Omar, Shuzan Ali Mohammed
Keywords
Not Available
Journal
Not Available
Publisher
Not Available
Volume
Not Available
Issue
Not Available
Pages
Not Available
publication.type
International
Paper Link
Not Available
Supplementary Materials
Not Available
Abstract
Background and Aim: Spontaneous bacterial peritonitis (SBP) is a prototypical infectious disease prevailing in patients with cirrhosis and ascites requiring speedy recognition and treatment. Once infection develops, proinflammatory and anti-inflammatory cytokines as (IL-10) are released to blood and ascites. This study aimed to evaluate the changes in serum and ascitic levels of Interleukin-10 in cirrhotic patients with spontaneous bacterial peritonitis to detect its role in diagnosis and/or pathogenesis of SBP.
Patients and Methods: We prospectively observed 65 subjects admitted in Hepatology, Gastroentrology and Infectious diseases Department, Benha university The subjects were categorized into three groups; group I included 20 healthy subjects (control group), group II included 20 cirrhotic patients without SBP and group III included 25 cirrhotic patients with SBP. IL-10 levels in the sera and ascitic fluids of studied groups were analyzed by ELISA and measured before and after treatment for SBP group.
Results: SBP was common in males (68%) with mean age was (55.76±9.46 years). Serum and ascitic IL-10 levels were higher in SBP group than non SBP and control groups with mean value was (67.51±34.03 vs 26.50±13.03, 17.08±5.20 pg/ml respectively) in serum and (209±98.46 vs 86.01±37.69 pg/ml respectively) in ascitic fluid with highly statistically significant difference between groups (P=0.0001). Moreover, SBP group showed significant reduction in the levels of IL-10 in blood and ascitic fluid after treatment. Serum IL-10 at cut off value (35.86 pg/ml) had high sensitivity, specificity, PPV and NPV (88%, 80%, 84.6%, 84.2% respectively) with AUC was (0.924) and (p=0.0001) and ascitic IL10 at cut off value (99.75 pg/ml) had high sensitivity (80%) and low specificity (65%) with AUC was (0.874) and (P =0.0001).
Conclusion: Serum IL-10 better than ascitic IL-10 in diagnosis of SBP, IL-10 levels in both serum and ascitic fluid may be related to clinical course of SBP.
Patients and Methods: We prospectively observed 65 subjects admitted in Hepatology, Gastroentrology and Infectious diseases Department, Benha university The subjects were categorized into three groups; group I included 20 healthy subjects (control group), group II included 20 cirrhotic patients without SBP and group III included 25 cirrhotic patients with SBP. IL-10 levels in the sera and ascitic fluids of studied groups were analyzed by ELISA and measured before and after treatment for SBP group.
Results: SBP was common in males (68%) with mean age was (55.76±9.46 years). Serum and ascitic IL-10 levels were higher in SBP group than non SBP and control groups with mean value was (67.51±34.03 vs 26.50±13.03, 17.08±5.20 pg/ml respectively) in serum and (209±98.46 vs 86.01±37.69 pg/ml respectively) in ascitic fluid with highly statistically significant difference between groups (P=0.0001). Moreover, SBP group showed significant reduction in the levels of IL-10 in blood and ascitic fluid after treatment. Serum IL-10 at cut off value (35.86 pg/ml) had high sensitivity, specificity, PPV and NPV (88%, 80%, 84.6%, 84.2% respectively) with AUC was (0.924) and (p=0.0001) and ascitic IL10 at cut off value (99.75 pg/ml) had high sensitivity (80%) and low specificity (65%) with AUC was (0.874) and (P =0.0001).
Conclusion: Serum IL-10 better than ascitic IL-10 in diagnosis of SBP, IL-10 levels in both serum and ascitic fluid may be related to clinical course of SBP.