Staff Members - Benha University
Isolation and Characterization of Zinc Tolerant Bacteria from Contaminated Sediments and Soils in Egypt
• 2018
Publication Information
Authors
Eman A. El-Alkshar; Hamed E. Abou-Aly; Taha A. Tewfike; Rasha M. El-Meihy; Ahmed M. Yousif
Keywords
Heavy metals, zinc, Alcaligenes faecalis, biosorption, antioxidant activities.
Journal
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publication.type
International
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Supplementary Materials
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Abstract
A total of 71 zinc tolerant bacteria were ,isolated from Four heavy metals contaminated sediments and soils in
Egypt, three sediment samples (Upstream, midstream, downstream Al-Rahawy drain, Giza Governorate) and two
agricultural soil samples (Al-Gabal Al-Asfar region and Kafr-Ilwan village, Qalubia Governorate). The minimum
inhibitory concentration (MIC) and maximum tolerance concentration (MTC) under zinc (Zn+2) concentrations
ranged between 500-4500 mg/l. The most zinc tolerant isolate UR45 with MIC 4500 mg/l and MTC 4000 mg/l
was identified by partial sequence of 16S rRNA genes as Alcaligenes faecalis MG257493.1 (UR45). Tolerance
of A. faecalis to cadmium (Cd2+), copper (Cu2+) and lead (Pb2+) under different concentrations (1000-4500 mg/l)
was evaluated. Growth of the strain and its biosorption activities (metal uptake, metal residual, metal biosorption)
at 1000 mg/l of Zn2+
, Cd2+
, Cu2+ and Pb2+ under different pH values (5-9) was studied. pH 8 was optimum for
growth in media supplemented with Zn2+ or Cu2+
. Whereas, the biosorption potentials were differed according to
the examined metal. Finally, enzymatic (catalase, peroxidase, polyphenol oxidase) and non-enzymatic (inhibition
2,2-DiPhenyl-2-Picrylhydrazyl hydrate (DPPH)) antioxidant activities of the A. faecalis MG257493.1 in presence
of four heavy metals individually or in mixture at 1000 and 1500 mg/l were estimated. The obtained data showed
that most antioxidant activities of the strain was increased with the increasing of heavy metals concentrations up to 1500 mg/l.
Egypt, three sediment samples (Upstream, midstream, downstream Al-Rahawy drain, Giza Governorate) and two
agricultural soil samples (Al-Gabal Al-Asfar region and Kafr-Ilwan village, Qalubia Governorate). The minimum
inhibitory concentration (MIC) and maximum tolerance concentration (MTC) under zinc (Zn+2) concentrations
ranged between 500-4500 mg/l. The most zinc tolerant isolate UR45 with MIC 4500 mg/l and MTC 4000 mg/l
was identified by partial sequence of 16S rRNA genes as Alcaligenes faecalis MG257493.1 (UR45). Tolerance
of A. faecalis to cadmium (Cd2+), copper (Cu2+) and lead (Pb2+) under different concentrations (1000-4500 mg/l)
was evaluated. Growth of the strain and its biosorption activities (metal uptake, metal residual, metal biosorption)
at 1000 mg/l of Zn2+
, Cd2+
, Cu2+ and Pb2+ under different pH values (5-9) was studied. pH 8 was optimum for
growth in media supplemented with Zn2+ or Cu2+
. Whereas, the biosorption potentials were differed according to
the examined metal. Finally, enzymatic (catalase, peroxidase, polyphenol oxidase) and non-enzymatic (inhibition
2,2-DiPhenyl-2-Picrylhydrazyl hydrate (DPPH)) antioxidant activities of the A. faecalis MG257493.1 in presence
of four heavy metals individually or in mixture at 1000 and 1500 mg/l were estimated. The obtained data showed
that most antioxidant activities of the strain was increased with the increasing of heavy metals concentrations up to 1500 mg/l.