This study was conducted from April 2003 to May 2004, at Tukh area, Kalu¬bia Governorate. Diseased buffaloes suffered from severe emaciation and pro¬nounced lameness. Also there was decrease of milk yield and depraved appetite. Blood picture showed a significant decrease in Hb, PCV, RBCS, while WBCS, MCV and MCH showed significant increase. Also, there was significant in¬crease in lymphocytes. On the other hand, Neutrophils, Basophils, Monocytes did not change significantly. Biochemical analysis for serum from cases with chronic hypophosphatemia indicated a significant decrease in serum inorganic phosphorus, sodium, potassium, iron, total iron binding capacity, copper and glucose. While serum calcium, magnesium and zinc did not change signif- cantly. There was a significant decrease in serum total proteins and albumin. Also serum enzyme activities showed a significant increase in serum ALP, AST and LDH, while ALT did not change significantly. Also serum urea and creatinine increased significantly. These Animals were successfully treated with Toldimfos 20% (Tonopltosphan) with vitamin D (Devarol). Also, anti-inflammatory as declofenac acid 2.5% was injected to control locomotor distur¬bance.