Staff Members - Benha University
FLOW CYTOMETRIC STUDY OF T-CELL SUBSETS AND COSTIMULATORY MOLECULES IN CLINICAL FORMS OF HUMAN SCHISTOSOMA MANSONI CHRONIC INFECTION
Benha M. J. • 2009
Publication Information
Authors
Manar S. Azab MD, Khaled N. El-Fayoumy MD*
Mustafa N. Neamatallah MD** and Tawfik EL- Adl MD***
Departments of Parasitology, Internal Medicine*, Medical Biochemistry **,
Faculty of Medicine, Mansoura University , Department of , Internal Medicine***,
Keywords
schistosomiasis; clinical forms; T-cell subsets.
Journal
Benha M. J.
Publisher
Mustafa N. Neamatallah
Volume
26
Issue
2
Pages
6
publication.type
International
Paper Link
Open Link
Supplementary Materials
Not Available
Abstract
Helminthic parasites cause widespread, persistent infections in humans.
Schistosomiasis mansoni infected patients being in a chronic immune-
activation state enabled us to investigate the effects of such immune
activation on immune responses. We performed by flow cytometry
a phenotypic analysis of peripheral blood T lymphocytes from 64 Schistosoma
mansoni infected patients, in different clinical forms of the chronic
disease. The main findings in the patient group in comparison with the
non-infected controls were: (i) decreased CD3, CD4 and CD8 lymphocyte
counts; (ii) elevated levels of activated T cells (CD4 expressing HLA-DR);
(iii) decreased numbers of CD28+ CD8+ lymphocytes. These findings support
the notion that chronic helminthic infections cause persistent immune
activation that result in hyporesponsiveness and anergy. Such impaired
immune functions may diminish the capacity of these individuals to cope
with infections and to generate cellular protective immunity after vaccination.
Schistosomiasis mansoni infected patients being in a chronic immune-
activation state enabled us to investigate the effects of such immune
activation on immune responses. We performed by flow cytometry
a phenotypic analysis of peripheral blood T lymphocytes from 64 Schistosoma
mansoni infected patients, in different clinical forms of the chronic
disease. The main findings in the patient group in comparison with the
non-infected controls were: (i) decreased CD3, CD4 and CD8 lymphocyte
counts; (ii) elevated levels of activated T cells (CD4 expressing HLA-DR);
(iii) decreased numbers of CD28+ CD8+ lymphocytes. These findings support
the notion that chronic helminthic infections cause persistent immune
activation that result in hyporesponsiveness and anergy. Such impaired
immune functions may diminish the capacity of these individuals to cope
with infections and to generate cellular protective immunity after vaccination.