Staff Members - Benha University
Serology and PCR–Based Techniques for Detection of Fusarium Wilt In Sesame" Annals of Agric. Sc., Moshtohor, 45(2): 587-596. (2007).
Annals of Agric. Sc., Moshtohor, • 2007
Publication Information
Authors
Mahdy, A.M.M.; Nawal, A. Eisa; Faten M. Abdel-Latif, El-Wakil, A.A. and El-Wakil, D.A.
Keywords
Sesame, Serological methods, Fusarium, PCR, Detection
Journal
Annals of Agric. Sc., Moshtohor,
Publisher
Not Available
Volume
45
Issue
2
Pages
587-596
publication.type
International
Paper Link
Not Available
Supplementary Materials
Not Available
Abstract
It is well known that serological methods are very useful for detecting
some plant pathogens. In this study, the antisera of five Fusarium spp., i.e., F.
moniliforme, F. solani, F. oxysporum, F. roseum and F. semitectum were used to
investigate the serological relationships between them. Results illustrated that,
there was a specificity as for F. oxysporum for infection with sesame cv. Giza-32
and sesame cv. Tushka-1. Polymerase chain reaction (PCR) detection of F.
oxysporum isolates using F1 & F2 primers, showed a positive reactions with ten
F oxysporum isolates. DNA extracts of F. solani, F. moniliforme and F.
oxysporum gave positive reactions compared with the positive control. PCR
technique was able to detect very low amount of nucleic acid extracted from F.
oxysporum in a dilutions ranged from 10"' to 10*9. In addition, rest Its showed that
the F1 & F2 primers were useful for detection of 12 Fusarium is dates collected
from different locations. It could be concluded as PCR using the two primers
would provide a powerful tool for detection of F. oxysporum isolates.
some plant pathogens. In this study, the antisera of five Fusarium spp., i.e., F.
moniliforme, F. solani, F. oxysporum, F. roseum and F. semitectum were used to
investigate the serological relationships between them. Results illustrated that,
there was a specificity as for F. oxysporum for infection with sesame cv. Giza-32
and sesame cv. Tushka-1. Polymerase chain reaction (PCR) detection of F.
oxysporum isolates using F1 & F2 primers, showed a positive reactions with ten
F oxysporum isolates. DNA extracts of F. solani, F. moniliforme and F.
oxysporum gave positive reactions compared with the positive control. PCR
technique was able to detect very low amount of nucleic acid extracted from F.
oxysporum in a dilutions ranged from 10"' to 10*9. In addition, rest Its showed that
the F1 & F2 primers were useful for detection of 12 Fusarium is dates collected
from different locations. It could be concluded as PCR using the two primers
would provide a powerful tool for detection of F. oxysporum isolates.