Staff Members - Benha University
Impact of Using Brilliant Cresyl Blue Stain on Oocyte and Embryo Selection
Egypt J Vet Sci • 2017
Publication Information
Authors
Hasb El-Naby AlA, Karima GhM, El-Raey M, Ahmed YF, Abou El-Roos MEA, Sosa GAM
Keywords
Not Available
Journal
Egypt J Vet Sci
Publisher
Not Available
Volume
48
Issue
(1)
Pages
: 43-51
publication.type
Local
Paper Link
Open Link
Supplementary Materials
Not Available
Abstract
T HE SUCCESS of in vitro embryo production is ultimately dependent on the number and
quality of cumulus-oocyte complexes harvested from ovaries. The morphological criteria
are routinely used for oocytes selection in most laboratories. There is brilliant cresyl blue stain
(BCB) test allowing the selection of developmentally competent oocytes which is a simple,
quick, economic and feasible protocol. BCB is known to be a non-invasive methodology that allows the selection of immature oocytes in several species. BCB test determine the intra cellular
activity of glucose-6-phosphate dehydrogenase (G6PDH) that gradually decreases its activity
as oocytes reach their growth phase. The using of BCB test before IVM is still controversial, so
this review describes the use of BCB staining test for oocytes and embryo selection to investigate
the impact of application of this stain in in-vitro maturation and embryo production in animals.
quality of cumulus-oocyte complexes harvested from ovaries. The morphological criteria
are routinely used for oocytes selection in most laboratories. There is brilliant cresyl blue stain
(BCB) test allowing the selection of developmentally competent oocytes which is a simple,
quick, economic and feasible protocol. BCB is known to be a non-invasive methodology that allows the selection of immature oocytes in several species. BCB test determine the intra cellular
activity of glucose-6-phosphate dehydrogenase (G6PDH) that gradually decreases its activity
as oocytes reach their growth phase. The using of BCB test before IVM is still controversial, so
this review describes the use of BCB staining test for oocytes and embryo selection to investigate
the impact of application of this stain in in-vitro maturation and embryo production in animals.