Staff Members - Benha University
Investigation of the Booroola gene in Egyptian ewes with different reproductive status using PCR-RFLP
Egyptian Journal of Veterinary Sciences • 2016
Publication Information
Authors
Hiam N. Ahmed, Karima Gh. M. Mahmoud, Mohamed M.M. Kandiel, Nermeen A. Helmy, S.S. Ibrahim, M.F. Nawito and O.E. Othman
Keywords
Sheep, Booroola (FecB) gene, Genotyping, Ultrasound
Journal
Egyptian Journal of Veterinary Sciences
Publisher
NIDOC-ASRT
Volume
47
Issue
1
Pages
1-12
publication.type
International
Paper Link
Open Link
Supplementary Materials
Not Available
Abstract
Ovulation can be regulated by a group of genes, termed as fecundity (Fec) genes. The aim of this study is to identify genetic
polymorphism in the Booroola (FecB) gene in Barki, Rahmani, and Ossimi sheep breeds with different physiological status diagnosed by ultrasound. Accordingly, animals were early classified into three groups: carrying single fetus, twin fetuses and non-pregnant by ultrasonographic examination. Demonstration of the fetal number was available as early as Day 35-40 post-mating transrectally, and trans-abdominally. The fetal viability was checked through heart examination with M-Mode. Genomic DNA was extracted from blood samples of the total number of sheep and two primers were used to
amplify 190 and 140 bp fragments of FecB gene. The amplified fragments were digested using Ava II restriction enzyme. All sheep groups were non carriers for the FecB mutation and gave a 190 bp band (++) and 140 bp band (Fec++) for primer 1 and 2, respectively. In conclusion, no genetic polymorphism was detected in the three Egyptian sheep breeds in relation to pregnancy with single or twin fetuses. The study could be continued to search for other major genes.
polymorphism in the Booroola (FecB) gene in Barki, Rahmani, and Ossimi sheep breeds with different physiological status diagnosed by ultrasound. Accordingly, animals were early classified into three groups: carrying single fetus, twin fetuses and non-pregnant by ultrasonographic examination. Demonstration of the fetal number was available as early as Day 35-40 post-mating transrectally, and trans-abdominally. The fetal viability was checked through heart examination with M-Mode. Genomic DNA was extracted from blood samples of the total number of sheep and two primers were used to
amplify 190 and 140 bp fragments of FecB gene. The amplified fragments were digested using Ava II restriction enzyme. All sheep groups were non carriers for the FecB mutation and gave a 190 bp band (++) and 140 bp band (Fec++) for primer 1 and 2, respectively. In conclusion, no genetic polymorphism was detected in the three Egyptian sheep breeds in relation to pregnancy with single or twin fetuses. The study could be continued to search for other major genes.