Staff Members - Benha University
Isolation and molecular identification of two novel cyanobacterial isolates obtained from a stressed aquatic system
Gene Reports • 2018
Publication Information
Authors
Omnia A.M. Badr, Ibrahim I.S. EL-Shawaf, Hoda A.S. El-Garhy, Mahmoud M.A. Moustafa
Keywords
Cyanobacteria
16S rRNA
S. aphanizomenoides
BLAST
Phylogenetic
Journal
Gene Reports
Publisher
Elsevier
Volume
13
Issue
Not Available
Pages
110-114
publication.type
International
Paper Link
Open Link
Supplementary Materials
Not Available
Abstract
Cyanobacteria are immense sources of several pharmacological compounds such as flavonoids and carotenoids
with anti-inflammatory and antioxidant activity. Two novel cyanobacterial isolates were isolated and identified
by sequencing of 16S rRNA gene. The results revealed that the two isolates are Sphaerospermopsis aphanizomenoides (KU212886.1) and Cronbergia siamensis (KY296358.1). The BLAST alignment showed that the nearest
NCBI deposited sequences were Cronbergia siamensis (NR153750.1) to C. siamensis (KY296358.1) and
Sphaerospermopsis aphanizomenoides (LN846950.1) to S. aphanizomenoides (KU212886.1) with identity ratio 94%
and 93%, respectively. The phylogenetic trees confirmed the same identity ratios on the roots of clades. The two
newly identified isolates could be new species with novel and unique characteristics.
with anti-inflammatory and antioxidant activity. Two novel cyanobacterial isolates were isolated and identified
by sequencing of 16S rRNA gene. The results revealed that the two isolates are Sphaerospermopsis aphanizomenoides (KU212886.1) and Cronbergia siamensis (KY296358.1). The BLAST alignment showed that the nearest
NCBI deposited sequences were Cronbergia siamensis (NR153750.1) to C. siamensis (KY296358.1) and
Sphaerospermopsis aphanizomenoides (LN846950.1) to S. aphanizomenoides (KU212886.1) with identity ratio 94%
and 93%, respectively. The phylogenetic trees confirmed the same identity ratios on the roots of clades. The two
newly identified isolates could be new species with novel and unique characteristics.