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Saprolegniosis in goldfish, Carassius auratus, associated with Saprolegnia parasitica; molecular characterization and electron microscopy
Egy. J. Aquac. • 2015
Publication Information
Authors
Adel A. Shaheen1, Amel M. El Asely*1, Abd EL Latif A. M. 1 ,Mahmoud M. A. Moustafa2 and Hosam E. Elsaied3
Keywords
Saprolegniosis, Carassius auratus, molecular characterization, Electron microscopy.
Journal
Egy. J. Aquac.
Publisher
Egy. J. Aquac.
Volume
5
Issue
2
Pages
1-12
publication.type
International
Paper Link
Not Available
Supplementary Materials
Not Available
Abstract
Saprolegnia strain was isolated from infected goldfish,Carassius auratus, fingerlings. Fish showed extensive hyphal growth on the skin, fins, gills and eyes. The isolate was molecular identified through 18S rRNA gene and internal transcript spacer, ITS, sequencing. The isolate showed 18S rRNA gene nucleotide identity, 91.6%, and ITS homology with Saprolegnia parasitica. Scanning electron microscopy was applied for evaluating the pathogenicity for the retrieved isolate; which proved its high virulence accompanied with the high mortality rate reaching 100% of the infected fingerlings.