Staff Members - Benha University
EFFECT OF AQUILEGIA VULGARIS L. (COLUMBINE) EXTRACT ON ASPERGILLUS FLA VUS CONTAMINATED CORN GRAINS (ZEA MAIZE L.) IN EGYPT
• 2008
Publication Information
Authors
Embaby, E.M.1 ; M.A. Swelim 2 ; M.M. Hazaa2; M.A. Abdel-Whhab 3
and E.A. El-Shahat 4
1. Plant Pathology Department, National Research Centre, Cairo, Egypt.
2. Botany Department, Faculty of Science, Benha University.
3. Food Toxicology and Contaminants
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publication.type
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Abstract
Contamination of dry corn grains (Zea maize L.) by moulds at five
Egyptian Governorates resulted 1580 fungal isolates belonging four fungal
genera: Aspergillus, Fusarium, Penicillium and Trichoderma. Agar plate
(PDA) method was enhanced than blotter test. Disinfected grains of maize gave
less fungal frequency than non-disinfected grains in both agar plate method and
blotter test. Also, disinfected seeds lead to higher percentage of germinated
seeds in comparison with non-disinfected seeds in either seed testing (agar
plate and blotter test). Penicillium sp. was the most fungal frequency with all
A f-firn-£»nt C n ; r k » * n n r f i t r » c a m n l p c £r\cwic±A T'vis*Visris)vmn en 11' K i r» h re*r>rrAc±A
UillVlWlll V » V. 1 UIV JUUI [JIVl) IVI1V T » ^V4 J JL t * I *t^C*W » / I I t^l I. J £S . TT 111VU 1 WV1 V4
37.98% and 36.08% respectively. Aspergillus was moderate fungal frequency
23.31% belonging 20.88% of A. flaws, 3.8% of A. niger and 0.63% of
Aspergillus spp. Fusarium oxysporum was less fungal frequency and gave
0.06%. Kafr-El-Shikh location was the most fungal frequency than other
Governorates followed by Sharkia sample, Qualubia and Beny Swief gave
moderate fungal frequency while, Gharbia was less fungal frequency. Also,
Gharbia samples was enhanced which recorded hundred percent of germination
seeds with disinfected and non-disinfected seeds. Mycotoxin production was
tested using thin layer chromatography (TLC) and HPLC cleared that only one
isolate (S 208) gave positive reaction of aflatoxins Bi and B2 . Neither Gj nor
G2 were detected. Determination of quantitatively aflatoxins using HPLC gave
1.97 |ig/g corn grains of AFBi and 0.15 |ig/g of AFB2 .
Aquilegia vulgaris L (Rununculaceae) (Columbine) extract was found to be
reduced the growth rate of A. flavus in comparison with control. Decreasing the
growth rates of A. flavus with increasing the concentration of plant extract
application. Growth rate of third cone. (1.5 ml) was greatly affected than
others. Also, the inhibition zone were increased with increasing the application
of pbnt extract.
Egyptian Governorates resulted 1580 fungal isolates belonging four fungal
genera: Aspergillus, Fusarium, Penicillium and Trichoderma. Agar plate
(PDA) method was enhanced than blotter test. Disinfected grains of maize gave
less fungal frequency than non-disinfected grains in both agar plate method and
blotter test. Also, disinfected seeds lead to higher percentage of germinated
seeds in comparison with non-disinfected seeds in either seed testing (agar
plate and blotter test). Penicillium sp. was the most fungal frequency with all
A f-firn-£»nt C n ; r k » * n n r f i t r » c a m n l p c £r\cwic±A T'vis*Visris)vmn en 11' K i r» h re*r>rrAc±A
UillVlWlll V » V. 1 UIV JUUI [JIVl) IVI1V T » ^V4 J JL t * I *t^C*W » / I I t^l I. J £S . TT 111VU 1 WV1 V4
37.98% and 36.08% respectively. Aspergillus was moderate fungal frequency
23.31% belonging 20.88% of A. flaws, 3.8% of A. niger and 0.63% of
Aspergillus spp. Fusarium oxysporum was less fungal frequency and gave
0.06%. Kafr-El-Shikh location was the most fungal frequency than other
Governorates followed by Sharkia sample, Qualubia and Beny Swief gave
moderate fungal frequency while, Gharbia was less fungal frequency. Also,
Gharbia samples was enhanced which recorded hundred percent of germination
seeds with disinfected and non-disinfected seeds. Mycotoxin production was
tested using thin layer chromatography (TLC) and HPLC cleared that only one
isolate (S 208) gave positive reaction of aflatoxins Bi and B2 . Neither Gj nor
G2 were detected. Determination of quantitatively aflatoxins using HPLC gave
1.97 |ig/g corn grains of AFBi and 0.15 |ig/g of AFB2 .
Aquilegia vulgaris L (Rununculaceae) (Columbine) extract was found to be
reduced the growth rate of A. flavus in comparison with control. Decreasing the
growth rates of A. flavus with increasing the concentration of plant extract
application. Growth rate of third cone. (1.5 ml) was greatly affected than
others. Also, the inhibition zone were increased with increasing the application
of pbnt extract.