Staff Members - Benha University
MOLECULAR CLONING AND EXPRESSION OF CHOLINE DEHYDROGENASE GENE FROM PSEUDOMONAS AERUGINOSA IN ESCHERICHIA COLIDH5A
• 2010
Publication Information
Authors
Rady, A.M.*; A.A.Diab! ; S.H.Abdel-Aziz2 ; MJM.Hazaa2 and
Kh.A.EL-Dougdoug3
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publication.type
Local
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Abstract
Choline dehydrogenase (CHDH) of Ps. aeruginosa EG. isolate was
showed to have enhanced hypersalinty. The genomic library of
Ps .aeruginosa was isolated and screened for CHDH enzyme gene, The
bet A gene code for CHDH enzyme cloned in PGEM-T easy vector and
showed potential positive clone when expressed in E.coli DH5a.The size
of gene insert with M.W. 1.7Kbp, When cut with restriction enzymes.
Pilot protein expression study showed specific protein band of M.W. 60
KDa when expressed in E.coli DH5a, That induced with IPTG and
isolated from parent strain, Finally it was demonstrated that the
expression of the betA gene in Ps.aeruginosa was induced by the
presence of salt, glucose, or choline precursor and IPTG inducer.
showed to have enhanced hypersalinty. The genomic library of
Ps .aeruginosa was isolated and screened for CHDH enzyme gene, The
bet A gene code for CHDH enzyme cloned in PGEM-T easy vector and
showed potential positive clone when expressed in E.coli DH5a.The size
of gene insert with M.W. 1.7Kbp, When cut with restriction enzymes.
Pilot protein expression study showed specific protein band of M.W. 60
KDa when expressed in E.coli DH5a, That induced with IPTG and
isolated from parent strain, Finally it was demonstrated that the
expression of the betA gene in Ps.aeruginosa was induced by the
presence of salt, glucose, or choline precursor and IPTG inducer.