Staff Members - Benha University
Genetic variation within Ushaar (Calotropis procera Ait (Ait) f.) genotypes using SDS-PAGE for protein and isozyme analysis
The fourth Conference of Sustainable Agricultural Development, Fayoum University, Faculty of Agriculture 20-22 October 2008. • 2008
Publication Information
Authors
Hassan, A. M.; El-Shawaf, I.I.S.; Bekhit, M. M. M.; El-Saied, F. M.
and Masoud, I. M.
Keywords
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Journal
The fourth Conference of Sustainable Agricultural Development, Fayoum University, Faculty of Agriculture 20-22 October 2008.
Publisher
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Volume
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Issue
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Pages
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publication.type
International
Paper Link
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Supplementary Materials
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Abstract
Genetic relationships were evaluated among three genotypes of the Ushaar
(Calotropis procera Ait (Ait) f.), collected from El Maghara, Shalateen and Sant
Kathrin by employing SDS-PAGE for seed protein and peroxidase, polyphenyl
oxidase and Alcohol dehydrogenase isozymes analysis. SDS-PAGE generated a
total of 12 bands of which nearly 50 % were polymorphic. Two positive bands
were detected in the Ushaar genotype from Shalateen at the molecular weights;
230.40 and 175.86 KDa. Moreover, two positive bands were found in the
Ushaar genotype from Sant Kathrin at the molecular weights; 168.33 and
148.68 KDa. On the other hand, two negative bands were detected in the Ushaar
genotype from Shalateen at the molecular weights; 82.06 and 76.86 KDa,
respectively. Identifiable polymorphic bands were detected from peroxidase,
polyphenyl oxidase and Alcohol dehydrogenase analysis. However, these
polymorphic markers clearly distinguished between the three genotypes from El
Maghara, Shalateen and Sant Kathrin regions. The Similarity indices and
dendrogram for the genetic distances of the combination between the SDSPAGE
protein and the three isozymes revealed that the highest similarity was
42.90 % between the Ushaar genotypes from Sant Kathrin and Shalateen
regions. Meanwhile, the lowest similarity index was between the Ushaar genotypes from El Maghara and Shalateen regions (28.60 %). The dendrogram
resulting from the combination of the two techniques separated the three Ushaar
genotypes into one cluster (including El Maghara and Sant Kathrin genotypes)
with the Shalateen genotype alone.
(Calotropis procera Ait (Ait) f.), collected from El Maghara, Shalateen and Sant
Kathrin by employing SDS-PAGE for seed protein and peroxidase, polyphenyl
oxidase and Alcohol dehydrogenase isozymes analysis. SDS-PAGE generated a
total of 12 bands of which nearly 50 % were polymorphic. Two positive bands
were detected in the Ushaar genotype from Shalateen at the molecular weights;
230.40 and 175.86 KDa. Moreover, two positive bands were found in the
Ushaar genotype from Sant Kathrin at the molecular weights; 168.33 and
148.68 KDa. On the other hand, two negative bands were detected in the Ushaar
genotype from Shalateen at the molecular weights; 82.06 and 76.86 KDa,
respectively. Identifiable polymorphic bands were detected from peroxidase,
polyphenyl oxidase and Alcohol dehydrogenase analysis. However, these
polymorphic markers clearly distinguished between the three genotypes from El
Maghara, Shalateen and Sant Kathrin regions. The Similarity indices and
dendrogram for the genetic distances of the combination between the SDSPAGE
protein and the three isozymes revealed that the highest similarity was
42.90 % between the Ushaar genotypes from Sant Kathrin and Shalateen
regions. Meanwhile, the lowest similarity index was between the Ushaar genotypes from El Maghara and Shalateen regions (28.60 %). The dendrogram
resulting from the combination of the two techniques separated the three Ushaar
genotypes into one cluster (including El Maghara and Sant Kathrin genotypes)
with the Shalateen genotype alone.