Sensitivity of seven tomato cultivars grown under glasshouse conditions in Almaty, Kazakhstan to wilt disease caused by Fusarium oxysporum f.sp. lycopersici, was evaluated. Evaluation results revealed that the tested cultivars could be classified into three groups: 1) Susceptible cultivars (Carolina Gold and Dona), 2) Highly resistant (EXP 1 and EXP 2) and moderately resistant (EXP3, EXP5 and EXP4). All investigated growth characters and fruit yield/plant were significantly lower in inoculated than un-inoculated plants of most tested tomato cultivars particularly Carolina Gold and Dona. Also, the results pronounced that the high resistant cultivars could be used as sources of resistance in the tomato breeding programs. In tram of early fruit yield production, the moderately resistant EXP4 was significantly better than the highly resistant cultivars EXP1 or EXP2, so it could be subjected to further tests to introduce it as commercial cultivar in Kazakhstan.