Staff Members - Benha University
Assessement of genetic variability among Fayoumi, Rhode Island Red and their crosses using RAPD-PCR
Benha Veterinary Medical Journal • 2013
Publication Information
Authors
Samar, H ., Hemeda, SA ., Sosa, G.A.M., Abo Shady Asmaa
Keywords
Not Available
Journal
Benha Veterinary Medical Journal
Publisher
Not Available
Volume
25
Issue
Not Available
Pages
232-239
publication.type
Local
Paper Link
Open Link
Supplementary Materials
Not Available
Abstract
This study was carried out to evaluate the genetic diversity and phylogenetic relationships using
randomly amplified polymorphic DNA (RAPD) in four chicken populations; Fayoumi × Fayoumi
(FF), Fayoumi × Rhode Island Red (FR), Rhode Island Red× Fayoumi (RF) and Rhode Island Red×
Rhode Island Red (RR). Fifteen decamer (RAPD) primers were screened on pooled genomic DNA of
four chicken populations produced a total of 154 bands. The number of polymorphic bands was 55.0
with an average of 3.66 per primer. The level of polymorphism among the four chicken genotypes
was 36.34%. Genetic similarity between crossbred populations (FR and RF) was (80.28%) while, the
similarity between the purebred populations (FF and RR) was (76%). RAPD data showed a clear
distinction of Fayoumi from RIR.
randomly amplified polymorphic DNA (RAPD) in four chicken populations; Fayoumi × Fayoumi
(FF), Fayoumi × Rhode Island Red (FR), Rhode Island Red× Fayoumi (RF) and Rhode Island Red×
Rhode Island Red (RR). Fifteen decamer (RAPD) primers were screened on pooled genomic DNA of
four chicken populations produced a total of 154 bands. The number of polymorphic bands was 55.0
with an average of 3.66 per primer. The level of polymorphism among the four chicken genotypes
was 36.34%. Genetic similarity between crossbred populations (FR and RF) was (80.28%) while, the
similarity between the purebred populations (FF and RR) was (76%). RAPD data showed a clear
distinction of Fayoumi from RIR.