Molecular studies on toxigenic strains of bacillus cereus isolated from some meat products.
BVMJ • 2015
Publication Information
Authors
1Ashraf A. Abd El Tawab,1Fatma I. El-Hofy,2Dalia F. Khater and 2YAHYA M. AL-Baaly
Keywords
meat products, B. cereus, identification
Journal
BVMJ
Publisher
Faculty of Vet.Med. Benha Univ.
Volume
29
Issue
1
Pages
129-133
publication.type
International
Paper Link
Not Available
Supplementary Materials
Not Available
Abstract
A total of 120 random samples of meat products including luncheon, sausage, minced meat and beef
burger (30 from each) were collected from different supermarkets in Gharbia Governorate. The collected
samples were transferred directly to the laboratory to be examined bacteriologically for the detection of
B. cereus. All samples were cultured at 37°C for 24 hours aerobically on selective media (PEMBA) for
isolation and purification and on sheep blood agar to observe the hemolysis properties. The results
revealed that the incidence of B. cereus was 20%, 36.37%, 40% and 56.67% in luncheon, beef burger,
sausage and Minced meat, respectively. The suspected colonies were examined for their colonial
morphology, microscopical examination, and biochemical reactions.VITEK2 BCL Card was used for
more identification of B. cereus strains. The public health hazards of the isolated strains were discussed.
burger (30 from each) were collected from different supermarkets in Gharbia Governorate. The collected
samples were transferred directly to the laboratory to be examined bacteriologically for the detection of
B. cereus. All samples were cultured at 37°C for 24 hours aerobically on selective media (PEMBA) for
isolation and purification and on sheep blood agar to observe the hemolysis properties. The results
revealed that the incidence of B. cereus was 20%, 36.37%, 40% and 56.67% in luncheon, beef burger,
sausage and Minced meat, respectively. The suspected colonies were examined for their colonial
morphology, microscopical examination, and biochemical reactions.VITEK2 BCL Card was used for
more identification of B. cereus strains. The public health hazards of the isolated strains were discussed.
Staff Members - Benha University