Staff Members - Benha University
Antibacterial activity of some medicinal plant oils against Escherichia coli and Salmonella species in -vitro
BVMJ • 2015
Publication Information
Authors
Ashraf A. Abd El Tawab1, Fatma I. El -Hofy1, Soad S. Belih2 and Mariam M. El Shemy2
Keywords
E.coli, Salmonella, Essential oils, MIC, MBC
Journal
BVMJ
Publisher
Faculty of Vet.Med. Benha Univ.
Volume
28
Issue
2
Pages
163-168
publication.type
International
Paper Link
Not Available
Supplementary Materials
Not Available
Abstract
A total of 100 samples were collected (75 samples from diseased broiler chickens and 25 samples from
recently dead broiler chickens). The samples were examined bacteriological, fifty two isolates of E.coli
(52%) and seven isolates of Salmonellae (7%) were found. ten random samples of E.coli were sero
grouped where five were E.coli O78, two O157 and untyped and seven isolates of salmonellae were
serotyped where three S.Enteritidis, one S.Charity, one S.Remiremont and two untyped . Antibacterial
activity of five medicinal plant oils from Eucalyptus, Mint, Cinnamon, Garlic and Thyme were evaluated
against the isolated strains using micro-titer plate to determine the minimum inhibitory concentration
(MIC) and minimum bactericidal concentration (MBC) for these oils. MIC results proved that for Mint,
Cinnamon, and Garlic oils were ranged from 5-5120 μg/ml, for Eucalyptus 20-5120 μg/ml and for
Thyme 5-2560 μg/ml. While results of MBC of Thyme and Cinnamon oils were ranged from 10-2560
μg/ml. but MBC of Eucalyptus ranged from 40-5120 μg, MBC of Mint and Garlic oils were ranged
from 5-2560μg |ml, 20-2560μg |ml respectively.
recently dead broiler chickens). The samples were examined bacteriological, fifty two isolates of E.coli
(52%) and seven isolates of Salmonellae (7%) were found. ten random samples of E.coli were sero
grouped where five were E.coli O78, two O157 and untyped and seven isolates of salmonellae were
serotyped where three S.Enteritidis, one S.Charity, one S.Remiremont and two untyped . Antibacterial
activity of five medicinal plant oils from Eucalyptus, Mint, Cinnamon, Garlic and Thyme were evaluated
against the isolated strains using micro-titer plate to determine the minimum inhibitory concentration
(MIC) and minimum bactericidal concentration (MBC) for these oils. MIC results proved that for Mint,
Cinnamon, and Garlic oils were ranged from 5-5120 μg/ml, for Eucalyptus 20-5120 μg/ml and for
Thyme 5-2560 μg/ml. While results of MBC of Thyme and Cinnamon oils were ranged from 10-2560
μg/ml. but MBC of Eucalyptus ranged from 40-5120 μg, MBC of Mint and Garlic oils were ranged
from 5-2560μg |ml, 20-2560μg |ml respectively.