This investigation was applied on 55 puppies of 2-4 months age to evaluate the efficacy of cell culture live attenuated canine parvovirus and canine distemper vaccines and inactivated cell culture rabies virus vaccine used singly or simultaneously.
The efficacy of these vaccines was determined by estimating the antibodies titer using serum neutralization and haemagglutination inhibition tests in puppies sera and the challenge of vaccinated animals with virulent strains of parvo and canine distemper viruses.
All vaccinated animals did not showed any signs of illness, also neutralizing and haemagglutination inhibition antibodies were detected in puppies sera from one week post vaccination (1WPV) and reached to a peak level at 3WPV. This level of antibodies was nearly the same in both animal groups vaccinated singly or simultaneously. After challenge, the vaccinated animals were not exhibited clinical signs related to either parvovirus or canine distemper virus infection while the challenged non vaccinated animals revealed typical clinical manifestation of canine distemper and parvovirus infection. The challenge against rabies did not done to avoid its hazard to workers
Finally it could be concluded that, the used vaccines were safe and potent and there was no difference in the immune response and rate of protection post-challenge when they used singly or simultaneously with each other. So it could be recommended to use the simultaneous vaccination to control canine distemper, canine parvovirus infection and rabies in Egypt saving time, efforts and cost.