A total of 412 milk samples were collected from clinically and subclinical mastitic cows (188, 224) respectively and examined bacteriologically for Staphylococcus aureus for the isolation rate and studying of the phenotypic characterization of the isolates. The results revealed that S.aureus was isolated in an incidence of 50% and17.5% from clinically mastitic and subclinically mastitic cows respectively. All Staphylococcus isolates showed symmetrically in their phenotypic characterization including cultural characters on different media. Vitek2 system succeeded in providing definitive identification results for gram positive bacterial by identification card (GP) were used for rapid and easy identification of Staphylococcus spp