Bovine babesiosis is a febrile, tick-borne disease of cattle and buffalo, caused by one or more protozoan parasites of the genus Babesia.The term Babesiosis refers to the acute form. In this study we selected animals from endemic area of Babesia bigemina infection that suffered from fever, emaciated, anemia, icterus and variable weight loss. The acute form is generally characterized by rapid growth and multiplication of the parasite in blood so diagnosis of babesiosis by microscopic examination is applicable in positive infected animals. These ones were distributed in three groups; each group consists of 50 animals and we selected twenty animals healthy (not infected) as control group. The infected animals of the first group was not treated as control positive, the second group was treated with imidocarb antibabesial drug and the third group was treated with imidocarb and Vit E as antioxidant. A comparison was made between the two methods of treatment by measuring erythrocytes antioxidant mechanisms representing in (G6PD, GR-ase,SOD and MDA) and in plasma (NO and trace element as antioxidant). Blood samples were collected from all animals from jugular vein: two samples, one on anticoagulant to measure erythrocytes antioxidant mechanisms and another to measure NO and trace elements. Results showed increased values of oxidant and decreased values of antioxidant in calves buffaloes suffered from parasitic infection as compared with healthy animals that especially for values of Nitric Oxide (NO) and Malondialdehyde (MDA). So, there was an increase in oxidative markers in animals infected with babisiosis while the levels of antioxidants such as superoxide dismutase(SOD), glutathione reductase (GR-ase); Glucose-6-Phosphate Dehydrogenase(G6PD) decreased, and some trace element showed changes as well (Cu). Addition of antioxidant (Vit E) to treatment decreased oxidative effect of infection and played an important role in increase of antioxidant.